Inquiry
Quality Antibodies Facilitate Your Discovery
Recombinant Protein Expression in Mammalian System
A mammalian host system is the preferred expression platform for producing human or other mammalian proteins with the most native structure and appropriate post-translational modifications. Thus, mammalian expression is the system best to recombinantly express a particular protein with functional biological activities.

Yurogen provides recombinant protein expression services using our featured mammalian transient expression system. All our protein expression scientists have strong structural biology backgrounds, offering state-of-the-art design for target proteins. Our service can be tailored to your specialized requirements, such as endotoxin removal, SEC-HPLC, cIEF analysis, glycosylation characterization, and polishing purification (size exclusive and ion exchange) etc.
Service Features
Highly efficient Electroporation Platform

Enhancing Transient Expression Yield

Comprehensive Protein QC Platform

Protein Mass Spectrometry, Purity by SEC-HPLC, Isoelectric Focusing Electrophoresis, and more

Large-Scale Production Capacity

Expression Ranging from Micrograms to Grams.

Procedure
Workflow
PhaseServiceDescriptionTimeline
Phase ITarget Protein Analysis

Conduct structural and biological analysis on the target protein, provide a comprehensive protein expression proposal including the selection of inserts, fusion protein tags, expression vectors, and strains.

2 business days
Phase IIGene synthesis and plasmid construction

  • Codon optimization and gene synthesis
  • Expression plasmid construction and sequencing
  • Delivery of sequencing report (Optional)

5-10 business days
Phase IIIaProtein Expression : Pilot expression

  • Perform pilot protein expression in a 3-5 mL system, including two different protein tags and two combinations of expression conditions
  • Evaluate protein expression, solubility, and yield by SDS-PAGE and/or WB analysis
  • Deliver protein pilot expression report

5 business days
Phase IIIbProtein Expression： Large-scale expression and purification

    Based on client’s requirements, perform scaled-up expression:

  • Soluble proteins: One-step affinity purification based on the selected tag.
  • Inclusion body proteins: Protein refolding and purification.
  • Quality Control of purified protein, including SDS-PAGE, WB (Optional), SEC-HPLC (Optional), capillary electrophoresis (Optional), and intact mass spectrometry analysis (Optional).

10-15 business days