Monoclonal Antibodies (RmAbs)
Typically, RmAbs have 10 – 100 times higher affinity than mouse monoclonal antibodies. On average, the KD values of RmAbs range from 10-10 up to 10-12 M
The development of primary and secondary antibody repertoires in rabbits possesses unique characteristics, such as gut-associated lymphoid tissues (GALT) B cell development and multiple selection procedures. These unique characteristics may account for the improved quality of the antibodies produced in rabbits [4,5]. The high specificity of RmAbs has clear edges in assays:
Improved assay sensitivity
Increased assay accuracy with decreased false positive rate
Lower assay noise on paraffin-embedded tissue samples--- ideal choice for IHC
Due to the reduced immunodominance, rabbit antigen presenting cells can present a broader range of epitopes than their mouse counterparts. Many less abundant proteins or epitopes can be well presented and recognized by rabbits. Rabbits are therefore able to generate high affinity antibodies against difficult epitopes, such as protein modifications, enzyme cleavage sites, or conformational epitopes.
Partially due to the expression of all three subclasses of CD1 proteins, the nonpeptidic epitope presenting in rabbits is more efficient and can handle broader targets than that in mice. As a result, rabbits are widely adopted as preferable hosts to generate better antibodies to small molecules and haptens, including small molecule drugs, steroid hormones, lipids, and glycolipids.
Rabbit IgG has extra disulfide bonds in the variable region of the heavy chain; an extra disulfide bond also exists between Vκ and Cκ. These extra disulfide bonds may result in the great stability and long shelf life of rabbit antibodies.
Pan R, Chen Y, Vaine M, Hu G, Wang S, Lu S, Kong XP. Emerg Microbes Infect. 2015 Jul;4(7)
Gendusa R, Scalia C, Buscone S, Cattoretti G. J Histochem Cytochem. 2014;62:519-531
Rossi S, Laurino L, Furlanetto A, Chinellato S, Orvieto E, Canal F, et al. Am J Clin Pathol. 2005;124:295-302
Rocha R, Nunes C, Rocha G, Oliveira F, Sanches F, Gobbi H. Pathol Res Pract. 2008;204:655-62
Vilches-Moure J, Ramos-Vara J. J Vet Diagn Invest. 2005;17:346-50