Key Features:
1. Uses a proprietary computational algorithm to predict hot-spot CDR residues and introduce mutations using ambiguous codons.
2. A novel small perturbation mutagenesis strategy for effective and efficient diversification of multiple CDRs.
3. Construction of scFv or Fab sub-libraries using massive microchip-synthesized oligonucleotides with large diversity and low redundancy.
4. Highly adaptive procedures to optimize experimental conditions for recombinant phage production, affinity-driving panning, binding ELISA, et al.
5. Flexible packages are available to meet customer requirements. You may choose to include/exclude however many stages in your final package (eg. Sanger or next-generation sequencing; single or combinational CDR library screening).
6. Significant affinity improvement can be achieved for most antibodies. We guarantee >4-fold affinity increase for single CDR library screening and >10-fold affinity increase for combinational CDR library screening.
Antibody Affinity Maturation


Material Requirement:
1. Target antibody sequences of VL and VH.
2. Antigen and assay protocol for measuring binding affinity (preferably ELISA).
Deliverables:
1. VL and VH sequences of 3 top-affinity variants in pUC19 vector.
2. Purified target antibody and 3 top-affinity variants (1mg each in human IgG1 format)
3. Study report including experiment protocols, purified antibody QC, binding affinity data.